Milieu Intérieur lead publications

Standardized high-dimensional spectral cytometry protocol and panels for whole blood immune phenotyping in clinical and translational studies
Dott, T. et al.
Abstract
Flow cytometry is the method of choice for immunophenotyping in the context of clinical, translational, and systems immunology studies. Among the latter, the Milieu Intérieur (MI) project aims at defining the boundaries of a healthy immune response to identify determinants of immune response variation. ...
Cytometry. Part A
2024 Feb;105(2):124-138
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Smoking changes adaptive immunity with persistent effects
Saint-André, V. et al.
Abstract
Individuals differ widely in their immune responses, with age, sex and genetic factors having major roles in this inherent variability1-6. However, the variables that drive such differences in cytokine secretion-a crucial component of the host response to immune challenges-remain poorly defined. Here we investigated 136 variables and ...
Nature
2024 Feb 14. Online ahead of print.
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Unveiling Candida albicans intestinal carriage in healthy volunteers: the role of micro- and mycobiota, diet, host genetics and immune response
Delavy, M. et al.
Abstract
Candida albicans is a commensal yeast present in the gut of most healthy individuals but with highly variable concentrations. However, little is known about the host factors that influence colonization densities. We investigated how microbiota, host lifestyle factors, and genetics could shape C. albicans intestinal carriage in 695 healthy individuals from the Milieu Intérieur cohort. ...
Gut Microbes
2023 Dec;15(2):2287618
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Integrated analysis of whole blood oxylipin and cytokine responses after bacterial, viral, and T cell stimulation reveals new immune networks
Villain, E. et al.
Abstract
Oxylipins are major immunomodulating mediators, yet studies of inflammation focus mainly on cytokines. Here, using a standardized whole-blood stimulation system, we characterized the oxylipin-driven inflammatory responses to various stimuli and their relationships with cytokine responses. ...
iScience
2023 Jul 18;26(8):107422
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Variability of Primary Sjögren's Syndrome Is Driven by Interferon-α and Interferon-α Blood Levels Are Associated With the Class II HLA-DQ Locus
Trutschel, D. et al.
Abstract
Primary Sjögren's syndrome (SS) is the second most frequent systemic autoimmune disease, affecting 0.1% of the general population. To characterize the molecular and clinical variabilities among patients with primary SS, we integrated transcriptomic, proteomic, cellular, and genetic data with clinical phenotypes in a cohort of 351 patients with primary SS. ...
Arthritis & Rheumatology
2022 Dec;74(12):1991-2002
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The immune factors driving DNA methylation variation in human blood
Bergstedt, J. et al.
Abstract
Epigenetic changes are required for normal development, yet the nature and respective contribution of factors that drive epigenetic variation in humans remain to be fully characterized. Here, we assessed how the blood DNA methylome of 884 adults is affected by DNA sequence variation, age, sex and 139 factors relating to life habits and immunity. ...
Nature Communications
2022 Oct 6;13(1):5895
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Early IFNβ secretion determines variable downstream IL-12p70 responses upon TLR4 activation
Posseme, C. et al.
Abstract
The interleukin-12 (IL-12) family comprises the only heterodimeric cytokines mediating diverse functional effects. We previously reported a striking bimodal IL-12p70 response to lipopolysaccharide (LPS) stimulation in healthy donors. Herein, we demonstrate that interferon β (IFNβ) is a major upstream determinant of IL-12p70 production, ...
Cell Reports
2022 Jun 28;39(13):110989.
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Defects in mucosal immunity and nasopharyngeal dysbiosis in HSC-transplanted SCID patients with IL2RG/JAK3 deficiency
Goncalves, P. et al.
Abstract
Both innate and adaptive lymphocytes have critical roles in mucosal defense that contain commensal microbial communities and protect against pathogen invasion. Here we characterize mucosal immunity in patients with severe combined immunodeficiency (SCID) receiving hematopoietic stem cell transplantation (HSCT) with or without myeloablation. ...
Blood
Blood. 2022 Apr 28; 139 (17):2585-2600
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Rhesus negative males have an enhanced IFN gamma-mediated immune response to influenza A virus
Sugrue, JA. et al.
Abstract
The Rhesus D antigen (RhD) has been associated with susceptibility to several viral infections. Reports suggest that RhD-negative individuals are better protected against infectious diseases and have overall better health. However, potential mechanisms contributing to these associations have not yet been defined. ...
Genes and Immunity
2022 Apr; 23(2):93-98
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Integrative genetic and immune cell analysis of plasma proteins in healthy donors identifies novel associations involving primary immune deficiency genes
Caron, B. et al.
Abstract
Blood plasma proteins play an important role in immune defense against pathogens, including cytokine signaling, the complement system, and the acute-phase response. Recent large-scale studies have reported genetic (i.e., protein quantitative trait loci, pQTLs) and non-genetic factors, such as age and sex, as major determinants to inter-individual variability in immune response variation. ...
Genome Medicine
2022 Mar 9;14(1):28
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Immune Profiling Enables Stratification of Patients With Active Tuberculosis Disease or Mycobacterium tuberculosis Infection
Duffy, D. et al.
Abstract
Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb) infection and is a major public health problem. Clinical challenges include the lack of a blood-based test for active disease. Current blood-based tests, such as QuantiFERON (QFT) do not distinguish active TB disease from asymptomatic ...
Clinical Infectious Disease
Volume 73 Issue 9 Page: E3398-E3408
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Associations between untargeted plasma metabolomic signatures and gut microbiota composition in the Milieu Interieur population of healthy adults
Partula, V. et al.
Abstract
Host-microbial co-metabolism products are being increasingly recognised to play important roles in physiological processes. However, studies undertaking a comprehensive approach to consider host-microbial metabolic relationships remain scarce. ...
British Journal of Nutrition
Volume 126 Issue 7 Page: 982-992
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Autoantibodies neutralizing type I IFNs are present in similar to 4% of uninfected individuals over 70 years old and account for similar to 20% of COVID-19 deaths
Bastard, P. et al.
Abstract
Circulating autoantibodies (auto-Abs) neutralizing high concentrations (10 ng/mL, in plasma diluted 1 to 10) of IFN-α and/or -ω are found in about 10% of patients with critical COVID-19 pneumonia, but not in subjects with asymptomatic infections. ...
Science Immunology
20 Aug 2021 Vol 6, Issue 62
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Immune response profiling of patients with spondyloarthritis reveals signalling networks mediating TNF-blocker function in vivo
Menegatti S. et al.
Abstract
Antitumour necrosis factor (TNF) therapy has revolutionised treatment of several chronic inflammatory diseases, including spondyloarthritis (SpA). However, TNF inhibitors (TNFi) are not effective in all patients and the biological basis for treatment failure remains unknown. ...
Annals of the Rheumatic Diseases
Volume: ‏ 8; Issue: ‏ 4; Pages: ‏ 475-486; Published: ‏ APR 2021
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Unveiling Interindividual Variability of Human Fibroblast Innate Immune Response Using Robust Cell-Based Protocols
Chansard A. et al.
Abstract
The LabEx Milieu Interieur (MI) project is a clinical study centered on the detailed characterization of the baseline and induced immune responses in blood samples from 1,000 healthy donors. Analyses of these samples has lay ground for seminal studies on the genetic and environmental determinants of immunologic variance in a healthy cohort population. ...
Frontiers in Immunology
Front Immunol. 2021 Jan 11:11:569331. eCollection 2020.
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Gut microbiome stability and dynamics in healthy donors and patients with non-gastrointestinal cancers
Byrd A. et al.
Abstract
As microbial therapeutics are increasingly being tested in diverse patient populations, it is essential to understand the host and environmental factors influencing the microbiome. ...
Journal of Experimental Medicine
Volume: ‏ 218; Issue: ‏ ;1Article Number: e20200606 ; Published:Jan 4 2021
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Autoantibodies against type I IFNs in patients with life-threatening COVID-19
Bastard, P. et al.
Abstract
Interindividual clinical variability in the course of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is vast. We report that at least 101 of 987 patients with life-threatening coronavirus disease 2019 (COVID-19) pneumonia had neutralizing immunoglobulin G (IgG) autoantibodies (auto-Abs) against interferon-ω ...
SCIENCE
23 Oct 2020: Vol. 370, Issue 6515, eabd4585
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Associations between consumption of dietary fibers and the risk of cardiovascular diseases, cancers, type 2 diabetes, and mortality in the prospective NutriNet-Sante cohort
Partula V. et al.
Abstract
Mounting evidence, yet with varying levels of proof, suggests that dietary fibers (DFs) may exert a protective role against various chronic diseases, but this might depend on the DF type and source. ...
Americal Journal of Clinical Nutrition
Volume: 112; Issue: 1; Pages: 195-207; Published: Jul 2020
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Antibody-coated microbiota in nasopharynx of healthy individuals and IVIg-treated patients with hypogammaglobulinemia
Goncalves, P. et al.
Abstract
Respiratory diseases are important causes of death, which collectively account for more than 1 in 10 of all deaths worldwide (World Health Organization). Pathobionts, potential pathogenic bacteria that under normal circumstances are present as harmless microorganisms, can be found embedded in the nasopharyngeal commensal microbiota. ...
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
Volume: 145; Issue: 6; Pages: 1686-+; Published: June 2020
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A comprehensive assessment of demographic, environmental, and host genetic associations with gut microbiome diversity in healthy individuals
Scepanovic, P. et al.
Abstract
The gut microbiome is an important determinant of human health. Its composition has been shown to be influenced by multiple environmental factors and likely by host genetic variation. ...
MICROBIOME
Volume: 7; Issue: 1; Article Number: 130; Published: SEP 13 2019
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Milieu Intérieur lead publications

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